Trends in HPLC – Wide pH-Range Columns and Capillary Columns

Authors

  • Barry E. Boyes
  • Angelika Gratzfeld-Hüsgen
  • Roland Weber

DOI:

https://doi.org/10.2533/chimia.2001.48

Keywords:

Capillary-hplc, High ph, Inert rp material, Lc-ms, Peptide

Abstract

The topic covers the current trends in HPLC column development to robust columns that extend the usable pH-range. Examples are given on how intelligent surface chemistry enables silica-based materials to be used in the pH-range 1 to 11.
The resulting packing materials combine the extreme mechanically stability of silica with an excellent chemical stability. Especially in the LC-MS community high pH-applications are becoming more popular, because of the problems that ion-pair reagents cause with this detection technique.
The life-science arena with small sample amounts demands smaller id columns i.e. capillary columns. Capillary-HPLC instruments are now commercially available and the presentation will shed some light on various capillary column applications.

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Published

2001-02-28

How to Cite

[1]
B. E. Boyes, A. Gratzfeld-Hüsgen, R. Weber, Chimia 2001, 55, 48, DOI: 10.2533/chimia.2001.48.