IMAPlate Based Miniature, High Sensitive, Rapid Screening Method for Detecting Bioengineered, Secreted Lipase Activities in Yeast Expression Systems

Authors

  • Michel A. Sciotti Hochschule für Life Sciences Fachhochschule Nordwestschweiz Gründenstrasse 40 CH-4132 Muttenz
  • Guo Jun Chen NCL New Concept Lab Eichenstrasse 22 CH-4313 Möhlin
  • Daniel Gygax Hochschule für Life Sciences Fachhochschule Nordwestschweiz Gründenstrasse 40 CH-4132 Muttenz;, Email: daniel.gygax@fhnw.ch

DOI:

https://doi.org/10.2533/chimia.2010.789

Keywords:

Enzymatic assay, Imaplate, Miniaturized reactor, Protein expression, Screen

Abstract

A spectrophotometric assay based on a miniaturized 96-well plate device (IMAPlate) enables a rapid and simple screening of bioengineered recombinant lipases expressed and secreted by Saccharomyces cerevisiae. Starting from a colony, the test delivers a quantitative estimation of enzymatic activity titer in 24 h or less with manual high throughput performances.
CHIMIA Vol. 64 No. 11 (2010): Swiss Biotech

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Published

2010-11-26

How to Cite

[1]
M. A. Sciotti, G. J. Chen, D. Gygax, Chimia 2010, 64, 789, DOI: 10.2533/chimia.2010.789.

Issue

Vol. 64 No. 11 (2010): Swiss Biotech

Section

Scientific Articles

License

Copyright (c) 2010 Swiss Chemical Society

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.