Thin-layer Chromatography–Nuclear Magnetic Resonance Spectroscopy – A Versatile Tool for Pharmaceutical and Natural Products Analysis

FH – HES Universities of Applied Sciences

Authors

  • Angelo Gössi University of Applied Sciences Northwestern Switzerland, School of Life Sciences, Institute for Chemistry and Bioanalytics, Gründenstrasse 40, CH-4132 Muttenz, Switzerland
  • Uta Scherer University of Applied Sciences Northwestern Switzerland, School of Life Sciences, Institute for Chemistry and Bioanalytics, Gründenstrasse 40, CH-4132 Muttenz, Switzerland
  • Götz Schlotterbeck University of Applied Sciences Northwestern Switzerland, School of Life Sciences, Institute for Chemistry and Bioanalytics, Gründenstrasse 40, CH-4132 Muttenz, Switzerland. goetz.schlotterbeck@fhnw.ch

DOI:

https://doi.org/10.2533/chimia.2012.347

Keywords:

Interfaces, Natural products, Nuclear magnetic resonance, Pharmaceutical analysis, Thin layer chromatography, Tlc-ms, Tlc-nmr

Abstract

Thin-layer chromatography (TLC) is a mature and very established technique, frequently used in many fields of applications ranging from natural product analysis to chemical or pharmaceutical applications. The introduction of a commercially available TLC–MS interface was a major step complementing the ease of use of TLC with structural elucidation power of mass spectrometry (MS). The TLC-MS interface simplifies the workflow dramatically to gain structural information directly from TLC separations. This article describes the potential of TLC-nuclear magnetic resonance spectroscopy (NMR) utilizing the TLC-MS interface to straightforwardly characterize zones of interest by NMR spectroscopy with a focus on quantification of active pharmaceutical ingredients (API) in formulations and identification of active principles in plant extracts.

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Published

2012-05-30

How to Cite

[1]
A. Gössi, U. Scherer, G. Schlotterbeck, Chimia 2012, 66, 347, DOI: 10.2533/chimia.2012.347.

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Section

Columns, Conference Reports

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