Construction of a Peptide Microarray for Auto-anti- body Detection
FH - HES
Keywords:Bard1, Click chemistry, Microarray, Peptide antigens, Tumor auto-antibodies
AbstractPeptide and protein microarrays provide a multiplex approach to identification and quantification of protein–protein interactions (PPI), useful to study for instance antigen–antibody properties. Multivariate serology assays detecting multiple tumor auto-antibodies (TAA) is an emerging class of blood tests for cancer detection. Here we describe the efficient coupling of peptide baits derived from the BRCA1-associated RING domain protein 1 (BARD1) to a solid surface and detection of a commercially available anti-BARD1 antibody with this newly designed peptide microarray. Analytical sensitivity and specificity were shown to be comparable to a microtiter plate based enzyme-linked immunosorbent assay (ELISA).
How to Cite
V. Cosandey, F. Debrot, J. Kaeser, R. Marti, P. Passeraub, J. Pétremand, D. Prim, M. E. Pfeifer, Chimia 2012, 66, 803, DOI: 10.2533/chimia.2012.803.
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