@article{Liberda_Trnka_Sejbal_Jonáková_Kraus_Tichá_1999, title={Preparation of Biotinylated and FITC-Labelled Phosphorylcholine Poly(acrylamide) Derivatives and Their Application for Protein Ligand-Binding Studies}, volume={53}, url={https://www.chimia.ch/chimia/article/view/1999_528}, DOI={10.2533/chimia.1999.528}, abstractNote={<p>Biotin- and FITC-labelled water-soluble poly(acrylamide) derivatives of phosphorylcholine were prepared by coupling either maleinylated (<em>a</em>) or periodate-oxidized (<em>b</em>) L-glyceryl phosphorylcholine to poly-(acrylamide-allylamine) copolymer. Biotinylated phosphorylcholine poly(acrylamide) derivatives of both types were tested with <em>Limulus polyphemus</em> C-reactive protein and were used for the study of the phosphorylcholine-binding properties of boar seminal plasma proteins. Binding sites for phosphorylcholine on the surface of bull sperms were visualized using a FITC-labelled derivative of the ligand.</p>}, number={11}, journal={CHIMIA}, author={Liberda, Jiří and Trnka, Tomáš and Sejbal, Jan and Jonáková, Věra and Kraus, Marek and Tichá, Marie}, year={1999}, month={Nov.}, pages={528} }