TY - JOUR AU - Liberda, Jiří AU - Trnka, Tomáš AU - Sejbal, Jan AU - Jonáková, Věra AU - Kraus, Marek AU - Tichá, Marie PY - 1999/11/24 Y2 - 2024/03/29 TI - Preparation of Biotinylated and FITC-Labelled Phosphorylcholine Poly(acrylamide) Derivatives and Their Application for Protein Ligand-Binding Studies JF - CHIMIA JA - Chimia VL - 53 IS - 11 SE - Scientific Articles DO - 10.2533/chimia.1999.528 UR - https://www.chimia.ch/chimia/article/view/1999_528 SP - 528 AB - <p>Biotin- and FITC-labelled water-soluble poly(acrylamide) derivatives of phosphorylcholine were prepared by coupling either maleinylated (<em>a</em>) or periodate-oxidized (<em>b</em>) L-glyceryl phosphorylcholine to poly-(acrylamide-allylamine) copolymer. Biotinylated phosphorylcholine poly(acrylamide) derivatives of both types were tested with <em>Limulus polyphemus</em> C-reactive protein and were used for the study of the phosphorylcholine-binding properties of boar seminal plasma proteins. Binding sites for phosphorylcholine on the surface of bull sperms were visualized using a FITC-labelled derivative of the ligand.</p> ER -