TY - JOUR AU - Pillet, Laurianne AU - Dufresne, Remy AU - Crelier, Simon PY - 2021/12/29 Y2 - 2024/03/29 TI - Copper-induced Production of Laccases for Lignin Depolymerisation and Micropollutant Degradation by Laccase-mediator Systems: FH-HES (Universities of Applied Sciences) JF - CHIMIA JA - Chimia VL - 75 IS - 12 SE - Columns, Conference Reports DO - 10.2533/chimia.2021.1058 UR - https://www.chimia.ch/chimia/article/view/2021_1058 SP - 1058 AB - <p>Contaminants deriving from human activities represent&nbsp;a constantly growing threat to our environment and have a direct&nbsp;impact on plant and animal health. To alleviate this ecological&nbsp;imbalance, biocatalysis offers a green and sustainable alternative&nbsp;to conventional chemical processes. Due to their broad&nbsp;specificity, laccases are enzymes possessing excellent potential&nbsp;for synthetic biotransformations in various fields as well as for&nbsp;the degradation of organic contaminants. Herein, we produced&nbsp;laccases in submerged cultures of P. ostreatus and T. versicolor&nbsp;in three different media. The fungi/medium combination leading&nbsp;to the highest enzymatic activity was malt extract (2%) + yeast&nbsp;extract (3%) + glucose (0.8%). Laccase production was further&nbsp;increased by supplementing this medium with different concentrations&nbsp;of Cu2+, which also provided a better understanding of&nbsp;the induction effect. Additionally, we disclose preliminary results&nbsp;on the interaction of laccases with mediators (ABTS and violuric&nbsp;acid - VA) for two main applications: lignin depolymerisation with&nbsp;guaiacylglycerol-?-guaiacyl ether (GBG) as lignin model and&nbsp;micropollutant degradation with Remazol Brilliant Blue (RBB)&nbsp;as enzymatic bioremediation model. Promising results were&nbsp;achieved using VA to increase depolymerization of GBG dimer&nbsp;and to enhance RBB decolorisation.</p> ER -